Photochemical Transfection: A Technology for Efficient Light-Directed Gene Delivery
Identifieur interne : 002194 ( Main/Exploration ); précédent : 002193; suivant : 002195Photochemical Transfection: A Technology for Efficient Light-Directed Gene Delivery
Auteurs : Anders H Gset [Norvège] ; Lina Prasmickaite [Norvège] ; Marit Hellum [Norvège] ; Birgit Enges Ter [Norvège] ; Vibeke M. Olsen [Norvège] ; Torunn E. Tjelle [Norvège] ; Carl J. Wheeler [États-Unis] ; Kristian Berg [Norvège]Source :
- Somatic Cell and Molecular Genetics [ 0740-7750 ] ; 2002-11-01.
Abstract
Abstract: Most synthetic gene delivery vectors are taken up in the cell by endocytosis, and inefficient escape of the transgene from endocytic vesicles often is a major barrier for gene transfer by such vectors. To improve endosomal release we have developed a new technology, named photochemical internalization (PCI). PCI is based on photochemical reactions initiated by photosensitizing compounds localized in endocytic vesicles, inducing rupture of these vesicles upon light exposure. PCI constitutes an efficient light-inducible gene transfer method in vivo, which potentially can be developed into a site-specific method for gene delivery in in vivo gene therapy. In this paper the principle behind the PCI technology and the effect of PCI on transfection with different synthetic gene delivery vectors are reviewed. PCI treatment by the photosensitizer aluminum phthalocyanine (AlPcS2a ) strongly improves transfection mediated by cationic polymers (e.g., poly-L-lysine and polyethylenimine), while the effect on transfection with cationic lipids is more variable. The timing of the light treatment relative to the transfection period was also important, indicating that release of the DNA from early endosomes is important for the outcome of PCI-induced transfection. The possibilities of using PCI as a technology for efficient, site-specific gene delivery in in vivo gene therapy is discussed.
Url:
DOI: 10.1023/A:1022979806314
Affiliations:
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To improve endosomal release we have developed a new technology, named photochemical internalization (PCI). PCI is based on photochemical reactions initiated by photosensitizing compounds localized in endocytic vesicles, inducing rupture of these vesicles upon light exposure. PCI constitutes an efficient light-inducible gene transfer method in vivo, which potentially can be developed into a site-specific method for gene delivery in in vivo gene therapy. In this paper the principle behind the PCI technology and the effect of PCI on transfection with different synthetic gene delivery vectors are reviewed. PCI treatment by the photosensitizer aluminum phthalocyanine (AlPcS2a ) strongly improves transfection mediated by cationic polymers (e.g., poly-L-lysine and polyethylenimine), while the effect on transfection with cationic lipids is more variable. The timing of the light treatment relative to the transfection period was also important, indicating that release of the DNA from early endosomes is important for the outcome of PCI-induced transfection. The possibilities of using PCI as a technology for efficient, site-specific gene delivery in in vivo gene therapy is discussed.</div></front></TEI><affiliations><list><country><li>Norvège</li><li>États-Unis</li></country><region><li>Californie</li><li>Østlandet</li></region><settlement><li>Oslo</li></settlement></list><tree><country name="Norvège"><region name="Østlandet"><name sortKey="H Gset, Anders" sort="H Gset, Anders" uniqKey="H Gset A" first="Anders" last="H Gset">Anders H Gset</name></region><name sortKey="Berg, Kristian" sort="Berg, Kristian" uniqKey="Berg K" first="Kristian" last="Berg">Kristian Berg</name><name sortKey="Enges Ter, Birgit" sort="Enges Ter, Birgit" uniqKey="Enges Ter B" first="Birgit" last="Enges Ter">Birgit Enges Ter</name><name sortKey="Enges Ter, Birgit" sort="Enges Ter, Birgit" uniqKey="Enges Ter B" first="Birgit" last="Enges Ter">Birgit Enges Ter</name><name sortKey="H Gset, Anders" sort="H Gset, Anders" uniqKey="H Gset A" first="Anders" last="H Gset">Anders H Gset</name><name sortKey="Hellum, Marit" sort="Hellum, Marit" uniqKey="Hellum M" first="Marit" last="Hellum">Marit Hellum</name><name sortKey="Olsen, Vibeke M" sort="Olsen, Vibeke M" uniqKey="Olsen V" first="Vibeke M." last="Olsen">Vibeke M. Olsen</name><name sortKey="Prasmickaite, Lina" sort="Prasmickaite, Lina" uniqKey="Prasmickaite L" first="Lina" last="Prasmickaite">Lina Prasmickaite</name><name sortKey="Tjelle, Torunn E" sort="Tjelle, Torunn E" uniqKey="Tjelle T" first="Torunn E." last="Tjelle">Torunn E. Tjelle</name></country><country name="États-Unis"><region name="Californie"><name sortKey="Wheeler, Carl J" sort="Wheeler, Carl J" uniqKey="Wheeler C" first="Carl J." last="Wheeler">Carl J. Wheeler</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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